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Content of modified nucleobases in HAP1 near-TKO state cells after exposition to vitamin C. Content of modified nucleobases in DNA isolated form HAP1 TET1KO/TET3KO cell line after 24 h of transfection with antiTET2 siRNA duplexes and exposition to 100 µmol/l of vitamin C. A 5-hmCyt; B 5-fCyt; C 5-caCyt, D 5-hmUra. Data presented as mean ± standard deviation, x: individual biological replicates. All datasets are available in article’s Additional file 1

Journal: Epigenetics & Chromatin

Article Title: Loss of TET2 activity limits the ability of vitamin C to activate DNA demethylation in human HAP1 cells

doi: 10.1186/s13072-025-00634-1

Figure Lengend Snippet: Content of modified nucleobases in HAP1 near-TKO state cells after exposition to vitamin C. Content of modified nucleobases in DNA isolated form HAP1 TET1KO/TET3KO cell line after 24 h of transfection with antiTET2 siRNA duplexes and exposition to 100 µmol/l of vitamin C. A 5-hmCyt; B 5-fCyt; C 5-caCyt, D 5-hmUra. Data presented as mean ± standard deviation, x: individual biological replicates. All datasets are available in article’s Additional file 1

Article Snippet: Three unique 27mer anti-TET2 siRNA duplexes (cat. No.: SR30002), Universal scrambled negative control siRNA duplex (cat. no.: SR30004) as well as siTran 2.0 transfection reagent (cat. no. TT320001) were bought from OriGene.

Techniques: Modification, Isolation, Transfection, Standard Deviation